Cell-Based Assays for Drug Discovery

Published by	Trimark Publications
Published	August, 2009	Product code	82871
Content info	103 pages
Price	US $ 3400 PDF by E-mail (Single User License) US $ 6800 PDF By E-mail (Unlimited User License)

Introduction
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Cell-Based Assays for Drug Discovery published by Trimark Publications in August, 2009. This report consists of 103 pages and the price starts from US $ 3400.

Introduction

Abstract

The area of drug discovery tools is one of the newest and most important sectors of pharmaceutical research and development. The term drug discovery tools usually refers to high-content screening (HCS) and analysis and is composed of those applications that require sufficient levels of sample throughput, whereby complex cellular events and phenotypes can be studied. Elements of drug performance like toxicity and specificity can be established simultaneously using mixed cell types-primary cells, cell lines, cell subpopulations. HCS seeks to assess the impact of phenotypic and cellular changes that are brought about by gene modification (such as with RNA interference (RNAi) approaches) and/or drug (or compound) treatment. The purpose of this examination by TriMark Publications is to describe the specific segments of the global drug discovery tools market. Within this area, the report covers those segments that are highly active in terms of innovation and growth. Specifically, this study examines the markets for small lab equipment all the way up to highly automated, large automated platforms, as well as accessory equipment such as reagents, supplies and manufacturers' original equipment manufacturer (OEM) additional equipment.

1. Overview

1.1 Objectives of the Report
1.2 Methodology
1.3 Scope of the Report
1.4 Executive Summary

2. Technologies and Product Offering for High-Content Analysis

2.1 Definition of High-Content Analysis and Why it is so Attractive a Discipline
2.2 Classes of Measurements Possible with High-Content Analysis Approaches and Biological Functions Investigated
2.3 Instrumentation Platforms for High-Content Analysis
- 2.3.1 High-Content Screening Technology
2.4 Reagent and Assay Platforms for High-Content Analysis
2.5 Cell-based Screening Technologies in Drug Development
- 2.5.1 Applications of Cell-based Assays
- 2.5.2 Pharma Drug Discovery Paradigm and Compound Screening
- 2.5.3 High-Content Analysis in the Biopharmaceutical Industry

3. Market Analysis of the High-Content Tools Space

3.1 High-Content Analysis Market Size and Growth
3.2 Market Survey to Assess Qualitative and Quantitative Parameters of the High-Content Analysis Space
3.3 Experimental and Research Trends in High-Content Analysis
3.4 Challenges and Market Drivers in High-Content Analysis
- 3.4.1 Barriers to High-Content Analysis
- 3.4.2 Drivers of High-Content Analysis
3.5 High-Content Analysis in Combination with RNAi
3.6 Market Landscape of Instrumentation for High-Content Analysis
3.7 Reagent and Assay Usage in High-Content Analysis
3.8 Trends in the High-Content Analysis Assays/Reagents Space-Major Product Vendors
3.9 Emerging Market Trends in High-Content Analysis
3.10 Market Forecasts for the High-Content Analysis Space
3.11 Use of HCS in Pharmaceutical Companies
3.12 Qualitative Opportunities and Challenges for Market Adoption

4. Strategic Analysis of the High-Content Tools Space

4.1 Analysis of the High-Content Market Structure
4.2 Description of the Drug Discovery Marketplace and Definition of the Field
4.3 Key Market Drivers and Challenges in the High-Content Analysis Space
4.4 Consolidated Picture of the High-Content Analysis Marketplace
4.5 High-Content Analysis Market Sectors and Growth Rate
4.6 Vendors of High-Content Analysis Technology

5. High-Content Analysis Technology Platforms

5.1 Methods of Digital Imaging
5.2 Fluorescence Microscopy
5.3 Major High-Content Analysis Instrumentation
- 5.3.1 High-Content Analysis Platforms to Support GPCR Screening
5.4 High-Content Analysis Reagents
5.5 Imaging Software
5.6 Enterprise-Level IT Solutions to Support High-Content Screening Experiments
- 5.6.1 Image Analysis Algorithms
5.7 Use of RNAi in High-Content Analysis
5.8 Industry Alliances to Leverage RNAi and High-Content Analysis
5.9 Emerging Trends in High-Content Analysis Technology Platforms

6. High-Content Analysis in Drug Selection, Screening and Biomarker Discovery

6.1 Stem Cells as Tools for Drug Discovery
6.2 Cellular Systems Biology for Development of Toxicity Panels in Drug Safety Testing
6.3 Drug Discovery Companies Marketing Cell-based Assays
6.4 Companies Using Cell-based Assays in Drug Discovery Programs
- 6.4.1 Phenotypic Drug Discovery (PDD)
- 6.4.2 Application of Quantitative High-Throughput Screening to HCA Cell-based Assays
- 6.4.3 Application of High-Content Fingerprinting to Oncology Drug Discovery: Focus on in vitro and in vivo Phenocopying and Cancer Stem Cell Analysis
6.5 Target Discovery and Validation by RNAi Screening
6.6 FLIM-FRET Methodology
6.7 Multidimensional Fluorescence Imaging (MDFI) Technology

7. Company Profiles

7.1 Acumen Pharmaceuticals, Inc.
7.2 Acumen Bioscience (division of TTP Group)
7.3 Applied Biosystems
7.4 BD Biosciences
7.5 DiscoveRX
7.6 Evotec Technologies (acquired by PerkinElmer)
7.7 Fisher BioImage
7.8 General Electric, GE Healthcare
7.9 Guava Technologies (now a division of Millipore)
7.10 Integral Molecular (Akceli Inc.)
7.11 LemnaTech GmbH
7.12 Millipore
7.13 Molecular Devices Corporation, now a division of MDS Analytical Techniques
7.14 NIH Chemical Genomics Center
7.15 PerkinElmer
7.16 Sangamo Biosciences, Inc.
7.17 Spotfire (acquired by TIBCO)
7.18 Thermo Scientific Cellomics
7.19 Translational Genomics Research Institute
7.20 Vala Sciences, Inc.

8. Glossary of Terms in the High-Content Analysis Space

LIST OF FIGURES

Figure 2.1: Scope of Biological Parameters Addressed via a Typical High-Content Analysis Experiment
Figure 2.2: Classes of Assays in Life Science Research and Drug Discovery Illustrating the Relationship between Cell-based Assays and High-Content Analysis
Figure 2.3: New Paradigm for Drug Discovery and Development Illustrating the Central and Essential Role of Screening
Figure 2.4: Cumulative Known and New Drug Targets
Figure 3.1: Breakout of Market Survey Respondents by Geographical Location
Figure 3.2: Breakout of Market Survey Respondents by Affiliation-Academic, Commercial, Vendor
Figure 3.3: Segmentation of Respondent Pool Based upon Usage of High-Content Analysis in its Research Activities
Figure 3.4: Segmentation of the Survey Respondent Pool Based upon the Length of Time they have been Using High-Content Analysis in Their Research Activities
Figure 3.5: Number of Parameters Studied Simultaneously in High-Content Analysis Assays-Multivariate (Multi-Parameter) Analyses
Figure 3.6: Key Biological Processes Studied Utilizing High-Content Analysis Tools
Figure 3.7: Breakout of High-Content Analysis Assays Currently Performed or Expected to be Performed in the Future by Biological Pathway (or Target)
Figure 3.8: Breakout of High-Content Analysis Experiments Performed Per Week (Distributed in our Respondent Pool) Across the Various Biological Pathways (and Targets)
Figure 3.9: Which of the Biological Processes (Pathways/Targets) Addressed Using High-Content Analysis-based Approaches are Growing in Importance and which are Declining?
Figure 3.10: In Which Environment are High-Content Analysis Assays Performed-Primary Screen, Secondary Screen, ADME/Tox Screen?
Figure 3.11: Key Challenges Faced by the Research Community in its Practice of High-Content Analysis
Figure 3.12: Various Drivers Leading the Research Community to Perform High-Content Analysis
Figure 3.13: HCA with RNAi-Current and Future Experimental Formats
Figure 3.14: HCA with RNAi-Number of Experiments Performed Per Month by the Survey Respondent Pool
Figure 3.15: Growing and Steady Usage of Various Formats where RNAi is coupled with HCA
Figure 3.16: Penetration of the Different High-Content Analysis Instrumentation Platforms into the Marketplace
Figure 3.17: Instrumentation Platforms for High-Content Analysis Ranked by Top Choice and Second Tier
Figure 3.18: High-Content Analysis Instrumentation and where they Lie on the Throughput Curve
Figure 3.19: Top Instrumentation Value Drivers in the High-Content Analysis Space
Figure 3.20: Important Sub-Cellular Features Studied via High-Content Analysis Approaches
Figure 3.21: Breakout of End-Point Versus Kinetic Assays in the High-Content Analysis Space
Figure 3.22: Types of Cellular Targets Studied Using High-Content Analysis Approaches
Figure 3.23: Top-most Target Class Studied Utilizing High-Content Analysis Approaches
Figure 3.24: Distribution of High-Content Analysis Experiments across the Respondent Pool-Number of Experiments Performed Per Week
Figure 3.25: Average Reagent/Assay Costs Per High-Content Analysis Experiment
Figure 3.26: Stratification of Reagent/Assay Suppliers into the High-Content Analysis Space
Figure 3.27: Monthly Reagent/Assay Purchases for High-Content Analysis by the End-user Community from Various Vendors
Figure 3.28: Growth or Decline in Importance of the Various High-Content Analysis Vendors to the End-user Community
Figure 3.29: Percentage of High-Content Analysis Experiments that Involve GFP across the Market Landscape
Figure 3.30: Breakout of High-Content Analysis Reagents Marketplace: Made-in-House Versus Off-the-Shelf
Figure 3.31: Breakout of Spending on Various Components of the High-Content Analysis Discipline
Figure 3.32: Historic/Forecast Growth of the Total Screening Space, Broken-out by Primary Screening, Secondary Screening (including High-Content Analysis as a Subset) and ADME/Tox, for 2004-2010
Figure 3.33: Historic/Forecast Growth of the Screening Space-Broken-out by Cell-based Assays and Biochemical Assays, 2004-2010
Figure 4.1: Drug Discovery and Development Ensemble and the Position of the Various Segments of High-Content Analysis in the Space
Figure 4.2: Relative Size and Position of the High-Content Analysis Space in the Overall Scheme of the Life Science Tools Marketplace
Figure 5.1: High-Content Analysis-Positional Biosensors Using Caspases and Monitoring the Translocation of a Tagged Protein from the Cytoplasm to the Nucleus
Figure 6.1: HCA Target Classes: Breakout of Current Drug Targets into Their Constituent Classes
Figure 6.2: GPCR Assay Technologies

LIST OF TABLES

Table 2.1: Comparison of the Key Features of High-Content Analysis and High-Throughput Screening
Table 2.2: Impact of High-Content Analysis on Drug Discovery-Impact of Several Drivers
Table 2.3: Biological Application Areas Associated with High-Content Analysis
Table 2.4: Classes of Measurements and Targets Identified Using Phenotypic Screening (High-Content Analysis)
Table 2.5: Classes of Cellular Measurements Possible with Fluorescent Protein Biosensors
Table 2.6: Multi-Parameter High-Content Analysis Assays to Study Biological Systems in Life Science Research and Drug Discovery, Demonstrating the Breadth and Scalability of the High-Content Analysis Approach
Table 2.7: Companies Offering Systems for High-Throughput Imaging
Table 2.8: Comparison of the Major Instrumentation Platforms and Their Associated Specifications for High-Content Analysis-I
Table 2.9: Comparison of the Major Instrumentation Platforms and Their Associated Specifications for High-Content Analysis-II
Table 2.10: Price Points and Target Markets of the Various High-Content Analysis Instrument Platforms
Table 2.11: Companies Offering Flow Cytometry Products and Services
Table 2.12: Integrated Product Platforms Offered by the Different High-Content Analysis Vendors
Table 2.13: High-Content Analysis Assays Developed by Thermo Scientific Cellomics
Table 2.14: Thermo Scientific Cellomics HCS Reagent Kits (formerly called "HitKits") and Their Therapeutic Areas of Application
Table 2.15: Millipore' s HCA Assay/Reagent Portfolio
Table 2.16: Cell Lines for GPCR High-Content Analysis that can be Deployed onto the BD Biosciences/Atto Pathway HT"! Instrument Platform
Table 2.17: Examples of High-Content Screens
Table 3.1: What Fraction of High-Content Analysis Assays are Cell-based Versus Biochemical-based
Table 4.1: Comparison of the Key Features of High-Content Analysis and High-Content Screening
Table 4.2: Snapshot of the Various HCA Assays, Demonstrating the Scalability of this Discipline
Table 5.1: Modes of Digital Imaging
Table 5.2: Modes of Fluorescence Microscopy
Table 5.3: Major High-Content Analysis Instrumentation
Table 5.4: Image Analysis Algorithms